Résumé
Wolfgang F. Bluhm, Ph.D.
Home Page
Techniques
COMPUTING AND ENGINEERING:
Perl, Java, C/C++, Fortran, SQL, relational databases (Sybase, Oracle, MySQL), (X)HTML, Unix/Linux, Mac, PC/Windows, bioinformatics software tools, computer modeling of cardiac physiology, data acquisition and analysis, instrumentation designPHYSIOLOGY:
Small animal surgery (rat aortic banding), contractile function of papillary muscles, force measurements in single cardiac myocytes, cell shortening, rapid cooling contracturesCELLULAR AND MOLECULAR BIOLOGY
Myocyte isolation, cell culture, immunocytochemistry, simulated ischemia, library screening, adenoviral gene transfer, Western blot, DNA preparation and analysis, cloningOPTICS AND IMAGING:
Confocal microscopy, light microscopy, image analysis, spectroscopy, interferometry, micromanipulation
Employment
(German Citizen, U.S. Permanent Resident)2001-present: Production Manager, Protein Data Bank (PDB), San Diego Supercomputer Center (SDSC)
- Managing data distribution and query functionality
- Managing development and implementation of new features
2000-2001: Application Developer, Protein Data Bank (PDB), San Diego Supercomputer Center (SDSC)
- Designed and implemented redundancy filter for PDB web interface
- Developed Perl modules for parsing mmCIF files (new standard in structural biology)
- Derived Swiss-Prot cross-reference consensus for data uniformity at PDB
1996-2000: Postgraduate Researcher, Department of Medicine, University of California, San Diego (UCSD)
- Cloned mouse HCN2 and Kv4.2 ion channel promoters (more)
- Evaluated approaches to cardiac in vivo gene therapy (more)
- Characterized contractile phenotypes of transgenic mice in isolated papillary muscles (more)
- Discovered important component of cardiac force-frequency relationship (more)
- Demonstrated ischemic protection of cytoskeleton by specific heat shock proteins (more)
1995-1996: Staff Volunteer, Medical Mission and Orphanage of Foundation for His Ministry, Vicente Guerrero, Baja California, Mexico
1986-1992: Various Teaching Assistantships
Education
1995: Ph.D., Bioengineering, University of California, San Diego
- Investigated mechanisms of cardiac length-tension relationship
- Measured contractile force in isolated mammalian cardiac myocytes
- Developed computational models of cardiac contractile function
1990: M.S., Engineering Sciences (Bioengineering), University of California, San Diego
1989: Diplom (similar to M.S.), Physics, University of Siegen, Germany
- Designed a Fourier spectrometer for high resolution spectral analysis of faint light sources
Awards
1998-2000: National Research Service Award (individual postdoctoral training grant), NIH
1992-1995: American Heart Association, California Affiliate, Predoctoral Research Fellow
1987-1990: German National Scholarship Foundation (supports top 1% of German students)
Languages
Bilingual in English and German, proficient in Spanish, knowledge of French
Comments or questions about this site: webmaster@wbluhm.com Copyright © 2006 Wolfgang Bluhm S.D.G.